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Carl Anderson, Ph.D.
Senior Scientist and Chair, Biology Department,
Brookhaven National Laboratory.
Funding through the National Cancer Institute, U.S. Army, and the Department
of Energy.
Figure 1. p53
ChIP-GST method for analyzing p53 binding sites in human genomes.
The human genome is a complex architecture of proteins and DNA
that together specify and regulate the expression of the information
necessary to specify life. Nornal human cells have ~88,500 predicted
p53 binding sites but only ~1000 – 10,000 p53 molecules.
To experimentally determine the actual sites with which p53
interacts under any give experimental or physiological condition
we have developed a new technique, ChIP-GST (Chromatin Immunoprecipitation
– Genome Signature Tags, that should be capable of identifying
and quanfying all of the sites in a genome with which a sequence-specific
binding protein, such as p53, actually interacts. |
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The p53 tumor suppressor is a tetrameric transcription factor that regulates
the expression of approximately 1,500 genes that control cell fate in
response to stress. Using a new method developed at BNL, Chromatin Immuno-Precipitation
Genome Signature Tags (ChIP-GST), the whole human genome will be analyzed
to determine the sites with which p53 interacts in normal cultured human
cells and after genotoxic stress. This analysis represents the first whole
genome analysis for functional interaction sites of any human sequence-specific
binding protein and will give, for the first time, a nearly complete set
of all sites in the human genome with which p53 binds.
A student with strong computational and/or mathematical background would
represent a welcome addition to this research program and would participate
in the development of algorithms to identify and classify the subset of
genomic sites with which p53 interacts as a function of the type of stress,
growth state, and cell type.
Contact Information
email:
url:
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