The objective of this research
is to develop highly sensitive assays for the detection of DNA damage
caused by reactive oxygen species (ROS). The term ROS includes hydroxyl
radical, hydrogen peroxide, and superoxide. These reactive species
are generated in vivo as the inadvertent consequences of normal
metabolic processes. Environmental conditions, such as ionizing
radiation or inappropriate diet, can also increase the in vivo level
of oxidative stress and lead to the same kinds of DNA damage. ROS
have been implicated in numerous human disabilities including cancer
and various neurodegenerative diseases. The mechanism of ROS involvement
in these diseases is most likely via DNA damage. There exists a
need for a technology that will accurately and quantitatively measure
the levels of oxidative DNA damage in cells and tissues. Assays
have been developed in this laboratory for measuring specific types
of ROS-induced DNA damage. These assays utilize 32P-postlabeling
as the method of detection and consequently are extremely sensitive.
Kits are to be manufactured that will facilitate the use of this
technology for the measurement of oxidative DNA damage. The measurements
are made unequivocal and quantitative by the inclusion in the kit
of appropriate carriers and internal standards. The availability
of these kits will make the assay practicable for most research
and clinical laboratories. A significant market for the kits is
anticipated in view of the important role oxidative stress has on
human health. |
